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| Registros recuperados : 14 | |
| 1. |  | LEASTRO, M. O.; PAZ LIMA, M. L.; SILVA, C. F. Aspectos morfofisiologicos de isolados de Myrothecium roridum. Tropical Plant Pathology, Brasília, DF, v. 34, p. S180, 2009. Suplemento. Edição dos Resumos do XLII do Congresso Brasileiro de Fitopatologia, Rio de Janeiro, RJ, 2009.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 3. | | LEASTRO, M. O.; KITAJIMA, E. W.; SILVA, M. S.; RESENDE, R. O.; ASTUA, J. de F. Dissecting the subcellular localization, intracellular trafficking, interactions, membrane association, and topology of Citrus Leprosis Virus. C proteins. Frontiers in Plant Science, v. 19, 2018.| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Recursos Genéticos e Biotecnologia. |
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| 5. | | MACEDO, M. A.; PEREIRA, T. G.; LEASTRO, M. O.; INOUE-NAGATA, A. K. Efeito de adubação e do hospedeiro na expressão de sintomas causados por begomovírus em plantas de tomate. Tropical Plant Pathology, Brasília, DF, v. 35, p. S281, ago. 2010. Suplemento. Resumo 11.050. Trabalho apresentado no 43. Congresso Brasileiro de Fitopatologia, 2010, Cuiabá.| Biblioteca(s): Embrapa Hortaliças. |
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| 6. | | LEASTRO, M. O.; OLIVEIRA, A. S.; LIMA, M. F.; RESENDE, R. O. Caracterização da proteína supressora de silenciamento gênico (NSs) do Zucchini lethal chlorosis. Tropical Plant Pathology, Brasília, DF, v. 36, 2011. Suplemento. Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves. Resumo 835.| Biblioteca(s): Embrapa Hortaliças. |
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| 10. | | HALLWASS, M.; LEASTRO, M. O.; LIMA, M. F.; INOUE-NAGATA, A. K.; RESENDE, R. O. Sequence determination and analysis of the NSs genes of two tospoviruses. Archives of Virology, New York, v. 157, n. 3, p. 591-596, Mar. 2012.| Biblioteca(s): Embrapa Hortaliças. |
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| 12. | | LEASTRO, M. O.; CASTRO, D. Y. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. Citrus LEPROSIS virus c codifica três proteínas com atividades supressoras de silenciamento gênico Citrus Leprosis Virus C encodes three proteins with gene silencing suppression activity. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 52., 2021. Anais... [Brasília, DF]: Sociedade Brasileira de Fitopatologia, 2021. p. 190. Evento online.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | LEASTRO, M. O.; VILLAR-ÁLVAREZ, D.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. Spontaneous mutation in the movement protein of Citrus Leprosis Virus C2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage. Viruses , v.13, n.12, 2021.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 14 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
20/01/2022 |
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Data da última atualização: |
20/01/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LEASTRO, M. O.; VILLAR-ÁLVAREZ, D.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. |
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Afiliação: |
MIKHAIL OLIVEIRA LEASTRO, Instituto Biológico, São Paulo; DAVID VILLAR-ÁLVAREZ, CSIC; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA, ESALQ; VICENTE PALLÁS, CSIC; JESÚS ÁNGEL SÁNCHEZ-NAVARRO, CSIC. |
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Título: |
Spontaneous mutation in the movement protein of Citrus Leprosis Virus C2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Viruses , v.13, n.12, 2021. |
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ISSN: |
1999-4915 |
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DOI: |
https://doi.org/10.3390/v13122498 |
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Idioma: |
Inglês |
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Conteúdo: |
Previous results using a movement defective alfalfa mosaic virus (AMV) vector revealed that citrus leprosis virus C (CiLV-C) movement protein (MP) generates a more efficient local movement, but not more systemic transport, than citrus leprosis virus C2 (CiLV-C2) MP, MPs belonging to two important viruses for the citrus industry. Here, competition experiment assays in transgenic tobacco plants (P12) between transcripts of AMV constructs expressing the cilevirus MPs, followed by several biological passages, showed the prevalence of the AMV construct carrying the CiLV-C2 MP. The analysis of AMV RNA 3 progeny recovered from P12 plant at the second viral passage revealed the presence of a mix of progeny encompassing the CiLV-C2 MP wild type (MPWT) and two variants carrying serines instead phenylalanines at positions 72 (MPS72F) or 259 (MPS259F), respectively. We evaluated the effects of each modified residue in virus replication, and cell-to-cell and long-distance movements. Results indicated that phenylalanine at position 259 favors viral cell-to-cell transport with an improvement in viral fitness, but has no effect on viral replication, whereas mutation at position 72 (MPS72F) has a penalty in the viral fitness. Our findings indicate that the prevalence of a viral population may be correlated with its greater efficiency in cell-to-cell and systemic movements. |
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Thesagro: |
Vírus. |
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Categoria do assunto: |
-- |
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Marc: |
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001 2139210
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022 $a1999-4915
024 7 $ahttps://doi.org/10.3390/v13122498$2DOI
100 1 $aLEASTRO, M. O.
245 $aSpontaneous mutation in the movement protein of Citrus Leprosis Virus C2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage.$h[electronic resource]
260 $c2021
520 $aPrevious results using a movement defective alfalfa mosaic virus (AMV) vector revealed that citrus leprosis virus C (CiLV-C) movement protein (MP) generates a more efficient local movement, but not more systemic transport, than citrus leprosis virus C2 (CiLV-C2) MP, MPs belonging to two important viruses for the citrus industry. Here, competition experiment assays in transgenic tobacco plants (P12) between transcripts of AMV constructs expressing the cilevirus MPs, followed by several biological passages, showed the prevalence of the AMV construct carrying the CiLV-C2 MP. The analysis of AMV RNA 3 progeny recovered from P12 plant at the second viral passage revealed the presence of a mix of progeny encompassing the CiLV-C2 MP wild type (MPWT) and two variants carrying serines instead phenylalanines at positions 72 (MPS72F) or 259 (MPS259F), respectively. We evaluated the effects of each modified residue in virus replication, and cell-to-cell and long-distance movements. Results indicated that phenylalanine at position 259 favors viral cell-to-cell transport with an improvement in viral fitness, but has no effect on viral replication, whereas mutation at position 72 (MPS72F) has a penalty in the viral fitness. Our findings indicate that the prevalence of a viral population may be correlated with its greater efficiency in cell-to-cell and systemic movements.
650 $aVírus
700 1 $aVILLAR-ÁLVAREZ, D.
700 1 $aASTUA, J. de F.
700 1 $aKITAJIMA, E. W.
700 1 $aPALLÁS, V.
700 1 $aSÁNCHEZ-NAVARRO, J. Á.
773 $tViruses$gv.13, n.12, 2021.
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